Flow Cytometry
About Our Flow Cytometry Laboratory
The Virant Diagnostics Flow Cytometry Laboratory is committed to offer patient-focused, informative, and impactful immune testing. We take pride in patient care with a focus on rapid turnaround time, expert interpretation reporting, and customizable testing solutions.
The Virant Diagnostics Flow Cytometry Laboratory offers standard immunophenotyping (SIP) and basophil activation testing (BAT). SIP allows in-depth immune system analysis; BAT, accounting for all characteristics of IgE and allergens, is an useful diagnostic modality in the diagnosis, treatment, and monitoring of various allergic conditions. Click the tabs below to learn more.
With immunophenotyping (SIP), we can identify and characterize variations of over- and/or under-expression of cells and cell-subsets of the immune system. Screening for immune disorders in patients with recurrent or unusual infections and monitoring immunodeficiencies are just some of the applications of SIP.
Our panel allows lymphocyte monitoring, evaluation of B cell maturation, and examination of memory T cells (CD4 and CD8) and dendritic cells. Click here to see the list of SIP markers.
The basophil is one of the white blood cells of the immune system that plays a key role in the mechanism of allergic inflammation. In vitro basophil activation from a blood sample can be more specific than traditional sensitization testing, and can therefore replace in vivo procedures (ie, intradermal testing; allergen challenge testing), of which the latter can result in allergic reactions of unpredictable severity. The former evaluates the allergic condition of the patients through basophil reactivity to allergens of varying titers.
With patient phenotype, Basophil Activation Test (BAT) can support the diagnosis of allergies (ie, food; drug; venom; etc) and chronic urticaria. BAT is also useful in the prediction and monitoring of clinical responses to immunomodulatory treatments (ie, allergen-specific immunotherapy and biologicals).
Our laboratory offers BAT for all common food allergies, environmental allergens, and venom. Check here to see the list of BAT allergens.
Currently for R&D only. Please contact us for more information.
- Lymphocyte Core Panel
- TBMNK Panel
- CD4+ T cell subset Panel
- Treg Panel
- Activated T cell Panel
- T cell senescence and exhaustion Panel
- T cell Inhibitory Receptor Panel
- B cell subset Panel
- NK cell Panel
- Dendritic cell Panel
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Resources
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Flow Cytometry Test Menu
Standard Immunophenotyping Test (SIP)
Included in the SIP Panel:
- Lymphocyte monitoring for:
- T cell subsets
- B & NK cells
- α/β & γ/δ T cells
- NKT cells & activation
- T cell memory
- B cell Maturation Evaluation: for B cell development in peripheral blood
- Memory CD8 and CD4 Cells:
- CD45RO
- CD45RA
- Dendritic Cell subtypes:
- CD11c
- BDCA2
Collection:
- Two (2) lavender-top EDTA tubes are needed, 3 mL minimum
- One (1) green-top tub, 7 mL minimum
Shipment:
- Ship specimens overnight with a cold pack.
Basophil Activation Testing (BAT)
- Egg white
- Milk
- Soybean
- Wheat
- Almond
- Brazil nut
- Cashew nut
- Hazelnut
- Pecan
- Peanut
- Pistachio
- Walnut (black)
- Walnut (English)
- Green pea
- Green bean
- Lima bean
- Navy bean
- Bass (black)
- Catfish
- Codfish
- Flounder
- Halibut
- Mackerel
- Perch
- Salmon
- Trout
- Tuna (yellowfin)
- Fish mix
- Mustard seed
- Sesame seed
- Clam
- Crab
- Crab (blue)
- Lobster
- Oyster
- Scallop
- Shrimp
- Shrimp (brown)
- Shellfish mix
- Barley
- Buckwheat
- Oat
- Rice
- Rye
- Broccoli
- Cabbage
- Carrot
- Cauliflower
- Celery
- Cucumber
- Lettuce
- Onion
- Pepper (black)
- Pepper (green)
- Potato (sweet)
- Potato (white)
- Spinach
- Squash (yellow)
- Tomato
- Apple
- Apricot
- Avocado
- Banana
- Blueberry
- Cantaloupe
- Cherry
- Coconut
- Cranberry
- Grapefruit
- Grape
- Lemon
- Olive
- Orange
- Peach
- Pear
- Pineapple
- Raspberry
- Strawberry
- Watermelon
- Cat
- Dog
- Dust mite
- Timothy grass
- Birch
- Oak
- Ragweed (s)
- Ragweed (L)
- Penecillin G
- Pre-pen
- Beef
- Chicken
- Lamb
- Pork
- Turkey
- Cocoa bean
- Cinnamon
- Coffee
- Garlic
- Ginger
- Hops
- Malt
- Mushroom
- Nutmeg
- Vanilla bean
A. BAT Screening: select up to 10 food and/or environmental allergens listed to the left. One concentration of allergen will be tested.
B. BAT Confirmation: select up to 3 food allergens listed to the left. Five concentrations of allergen will be tested.
C. Venom Panel: Five allergens in this panel:
- Yellow jacket
- Wasp
- Yellow hornet
- White faced hornet
- Honeybee
Collection:
- Two (2) lavender-top EDTA tubes are needed, 3 mL minimum
- One (1) green-top tub, 7 mL minimum
Shipment:
- Ship specimens overnight with a cold pack.
For more information on Allergies and our Allergy Testing Solutions:
Allergy testing frequently asked question (FAQs) for patients – Click the link for more information on basophil activation testing & ImmunoCAP allergy testing.